An assay includes catalytic hybridization of targets and cleavable probes to
form
triplexes and quadruplexes based on Watson-Crick bonding rules. The probes contain
scissile linkages that are cleaved by enzymes when hybridized to a target, yielding
detectable probe fragments free of the target. The target is recycled to help catalyze
the cleavage of additional intact probes to form additional detectable probe fragments,
thus amplifying the signal.
