The present invention relates to mutations of a subtilisin gene which result
in changes in the chemical characteristics of subtilisin enzymes. Mutations at
specific nucleic acids of the subtilisin gene result in amino acid substitutions
and consequently, altered enzyme function. Some of these mutant enzymes exhibit
physical properties advantageous to industrial applications, particularly in the
detergent industry, providing subtilisin which is more stable to oxidation, possesses
greater protease activity, and exhibits improved washability.
