The invention provides methods and systems for monitoring effects of chemical
agents on optical signals produced by samples in response to the chemical agents.
Preferred methods comprise application of multiple chemical agents that interact
to alter an optical signal from the sample. Methods and systems of the invention
also comprise monitoring an optical signal from an endogenous chromophore upon
application of a chemical agent to a sample. Methods and systems of the invention
also comprise the use of triggers, atomizers and image alignment to enhance the
results of methods described herein.
