A chimeric phosphorylation indicator is provided. A chimeric phosphorylation
indicator
can contain a donor molecule, a phosphorylatable domain, a phosphoaminoacid binding
domain (PAABD), and an acceptor molecule. A chimeric phosphorylation indicator
also can contain a phosphorylatable polypeptide and a fluorescent protein, wherein
the phosphorylatable polypeptide is contained within the sequence of the fluorescent
protein, or wherein the fluorescent protein is contained within the sequence of
the phosphorylatable polypeptide. Also provided are polynucleotides encoding such
chimeric phosphorylation indicators, as well as kits containing the indicators
or the polynucleotides. In addition, a method of using the chimeric phosphorylation
indicators to detect a kinase or phosphatase in a sample is provided.
