The present invention relates to a method for profiling of protein extract from
hyper-acidic aerial parts of plant Scented Geranium (Pelargonium sp.), said
method comprising steps of selecting effective extraction medium, with extraction
medium consisting of 0.2 M sodium carbonate increasing percentage extract by about
600%, selecting effective medium to determine polypeptide pattern in said extract,
with polypeptide pattern medium consisting of Sodium Carbonate ranging between
0.2 to 0.4 M, and 0.2 M Sodium Carbonate along with 50% dimethylsulfoxide, selecting
effective media to determine enzyme multimolecular forms in said extract, with
enzyme multimolecular forms media consisting of 0.2 M Tris-HCl buffer at pH 7.5,
and optionally 50% dimethylsulfoxide; Sodium Carbonate at concentration ranging
between 0.2 to 0.4 M and optionally 50% dimethylsulfoxide, and using biochemical
and biophysical techniques comprising gel electrophoresis, spectrophotometry, centrifugation,
protein estimation in said method to determine quantitative, qualitative, structural
and functional facets of the proteins from said extract, and extraction compositions thereof.
