The gammaglobulin is extracted from a fraction isolated by fractionation
with ethanol in the presence of a carbohydrate, and after reducing the
content of contaminants with PEG, it is applied to an anionic resin
exchange column, an effluent being obtained in which the PEG content is
subsequently reduced by ultrafiltration and which is concentrated in order
to carry out sequentially an optional treatment at an acid pH and at least
one of the following steps of viral inactivation, consisting of
pasteurisation and a treatment with solvent/detergent, the product
afterwards being precipitated and washed with PEG in order to eliminate
any chemical viral inactivation reagents and then, by solubilisation and
change of pH, the protein contaminants, and finally purified by
ultrafiltration to reduce the volume and the PEG content, then carrying
out an optional virus filtration and subsequent concentration.
