Methods and systems for detecting MHC class I and II binding peptides

   
   

The invention is based on the discovery that MHC class I and class II monomers immobilized to a solid surface are still capable of forming complexes with suitable MHC-binding peptides. Methods for detecting peptide binding to HLA monomers, and methods for measuring the relative degree of binding between two MHC-binding peptides as well as a method of measuring the rate of dissociation of peptides from MHC complexes are provided. The present invention also provides systems and kits useful for conducting the methods of the invention.

De uitvinding is gebaseerd op de ontdekking dat MHC de klasse I en klasse II monomeren die aan een stevige oppervlakte worden geïmmobiliseerd complexen met geschikte mhc-Bindende peptides nog kunnen vormen. De methodes om peptide te ontdekken die aan monomeren HLA bindt, en de methodes om de relatieve graad van band tussen twee mhc-Bindende peptides evenals een methode te meten om het tarief van scheiding van peptides van complexen te meten MHC worden verstrekt. De onderhavige uitvinding verstrekt systemen en uitrustingen ook nuttig om de methodes van de uitvinding te leiden.

 
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