Methods for stable transformation of plants

   
   

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.

Se induce a las estructuras múltiples del lanzamiento de los tejidos finos de planta (e.g., los ápices del lanzamiento o brotes axilares en un medio artificial) que produzcan culturas múltiples del lanzamiento. Este multi-tire a las culturas entonces son transformados por métodos sabidos de la transformación. Las plantas se regeneran posteriormente de las células transformadas. Las cosechas que pueden ser transformadas eficientemente con este método incluyen las plantas normalmente recalcitrant a la transformación tal como remolacha, girasol, soja, algodón, tabaco, tomate, cacahuetes, melones, sandía, calabaza, brassica, y pimienta.

 
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