Hyperactive light signal related molecule of HFR1-deltaN105 and transgenic plant thereof

   
   

This invention is about the functionally hyperactive light signal related molecule, HFR1-.DELTA.N105, of which the nucleic acids that encode N-terminal 105 amino acid residues were deleted. HFR1 as a bHLH transcription factor functions in a subset of phytochrome A signaling cascade and it was reported to be regulated negatively by COP1. Experiments with a HFR1-.DELTA.N105 overexpressing plant revealed that the deletion of N-terminal amino acids makes the HFR1 more active in photomorphogenic development such as germination and de-etiolation. In addition, the transgenic plants showed hypersensitive photo-responses in the inhibition of hypocotyl elongation, dependently on another positive element of light signaling, a bZIP protein, HY5. The end-of-day far-red light response and petiole elongation were suppressed in the HFR1-.DELTA.N105 overexpressing plants. These results suggest that N-terminal region of HFR1 negatively regulate HFR1 function and that HFR1-.DELTA.N105 is hyperactive.

Esta invenção é sobre sinal claro funcionalmente hyperactive a molécula relacionada, HFR1-.DELTA.N105, de que os ácidos nucleic que codificam resíduos do amino-ácido do N-terminal 105 foram suprimidos. HFR1 como funções de um fator da transcrição do bHLH em um subconjunto do phytochrome A que sinaliza a cascata e a foi relatado para ser regulado negativamente por COP1. As experiências com uma planta HFR1-.DELTA.N105 overexpressing revelaram que o apagamento de aminos-ácido do N-terminal faz o HFR1 mais ativo no desenvolvimento photomorphogenic tal como o germination e o de-etiolation-etiolation. Além, as plantas transgenic mostraram foto-respostas hypersensitive na inibição do elongation do hypocotyl, dependente de um outro elemento positivo de sinalizar claro, uma proteína do bZIP, HY5. O elongation claro distante-vermelho da resposta e do petiole de extremidade-$$$-DIA foi suprimido nas plantas HFR1-.DELTA.N105 overexpressing. Estes resultados sugerem que a região do N-terminal de HFR1 regula negativamente a função HFR1 e que HFR1-.DELTA.N105 é hyperactive.

 
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