Genetically-encodable, environmentally-responsive fusion proteins
comprising ELP peptides. Such fusion proteins exhibit unique
physico-chemical and functional properties that can be modulated as a
function of solution environment. The invention also provides methods for
purifying the FPs, which take advantage of these unique properties,
including high-throughput purification methods that produce high yields
(e.g., milligram levels) of purified proteins, thereby yielding sufficient
purified product for multiple assays and analyses. The high throughput
purification technique is simpler and less expensive than current
commercial high throughput purification methods, since it requires only
one transfer of purification intermediates to a new multiwell plate.
