The present invention relates to a method for producing a recombinant
adeno-associated virus (rAAV), in which at different times a helper
construct and also a vector construct are introduced into a suitable cell,
and preferably the helper construct does not comprise, in particular with
the exception of the AAV promoters, any nucleic acid sequences to which at
least a Rep protein can essentially specifically bind and preferably the
vector construct comprises ITR sequences in flop orientation. The
recombinant adeno-associated viruses produced according to the method of
the invention are suitable in particular for producing a tumor cell into
which additionally nucleic acids coding for GM-CSF and B7.2 has been
introduced, which in turn can be used in the form of a medicament for the
treatment of cancers.
