The present invention relates to a high-yield process for producing
high-activity, high-purity transglutaminase by refolding denatured
microorganism-derived transglutaminase by reacting denatured
transglutaminase in an aqueous medium under acidic conditions and
reconstituting a higher-order enzymatically active state in an aqueous
medium at a neutral pH.
La presente invenzione riguarda un alto procedimento del rendimento per produrre il transglutaminase high-activity e di grande purezza refolding il transglutaminase microorganismo-derivato denaturato reagendo il transglutaminase denaturato in un mezzo acquoso nelle circostanze silicee e ricostituendo enzimaticamente un attivo higher-order dichiari in un mezzo acquoso ad un pH neutro.
