This invention provides prokaryotic glycosyltransferases, including a
bifunctional sialyltransferase that has both an .alpha.2,3- and an
.alpha.2,8-activity. A .beta.1,4-GalNAc transferase and a
.beta.1,3-galactosyltransferase are also provided by the invention, as are
other glycosyltransferases and enzymes involved in synthesis of
lipooligosaccharide (LOS). The glycosyltransferases can be obtained from,
for example, Campylobacter species, including C. jejuni. In additional
embodiments, the invention provides nucleic acids that encode the
glycosyltransferases, as well as expression vectors and host cells for
expressing the glycosyltransferases.
