The present invention relates to a method of generating a separation
medium comprising mixed mode cation-exchanger ligands coupled to a base
matrix, which method comprises to provide a scaffold comprising a
functional group and exhibiting a cyclic core structure; derivatise the
scaffold with a reagent comprising a reactive group coupled to a residue
R by reacting the functional group of the scaffold with said reactive
group; open the cyclic structure of the resulting derivative; and react
the product with a base matrix comprising a reactive group. The scaffold
presents at least two functionalities; one sulphur-comprising group for
coupling to the base matrix and one group that can be transformed into an
ionic group.
