Methods and compositions for enhancing sensitivity in the analysis of biological-based assays

   
   

Methods are provided for detecting the binding of a first member to a second member of a ligand pair, comprising the steps of (a) combining a set of first tagged members with a biological sample which may contain one or more second members, under conditions, and for a time sufficient to permit binding of a first member to a second member, wherein said tag is correlative with a particular first member and detectable by non-fluorescent spectrometry, or potentiometry, (b) separating bound first and second members from unbound members, (c) cleaving the tag from the tagged first member, and (d) detecting the tag by non-fluorescent spectrometry, or potentiometry, and therefrom detecting the binding of the first member to the second member.

Os métodos são fornecidos detectando o emperramento de um primeiro membro a um segundo membro de um par do ligand, compreendendo as etapas (a) que combinam de um jogo de primeiros membros etiquetados com uma amostra biológica que possa conter aquele ou mais segundos membros, sob circunstâncias, e por um momento suficiente permitir ligar de um primeiro membro de um segundo membro, wherein o Tag dito é correlativo com um primeiro membro particular e detectável pelo spectrometry non-fluorescent, ou ao potentiometry, (b) separando o limite primeiramente e os segundos membros dos membros unbound, (c) cleaving o Tag do primeiro membro etiquetado, e (d) detectando o Tag o spectrometry non-fluorescent, ou o potentiometry, e therefrom detectar o emperramento do primeiro membro ao segundo membro.

 
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