High efficiency mRNA isolation methods and compositions

   
   

The present invention provides methods and compositions, including kits, for the isolation and purification of mRNA, particularly poly(A) RNA. It concerns the use of isostabilizing salts such as TMAC and TEAC to reduce rRNA carryover during the purification process, thus facilitating the isolation of poly(A) RNA.

Присытствыющий вымысел обеспечивает методы и составы, включая наборы, для изоляции и очищения mRNA, определенно RNA poly(A). Он относится польза isostabilizing соли such as TMAC и TEAC для уменьшения carryover rRNA во время процесса очищения, таким образом облегчающ изоляцию RNA poly(A).

 
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