Method of separating components in a sample using silane-treated silica filter media

   
   

The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment of the invention, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quarternary ammonium group or functional sulphonate group. Particulates suitable for the present invention, for example, are microorganisms.

A invenção atual fornece métodos separando um ou mais componente do interesse de uma amostra que contem particulates e materiais soluble. O método compreende as etapas de: (a) filtrando uma amostra com os meios do filtro do silicone cujo o silanol de superfície agrupa reagiu com um ou mais silanes, e (b) simultaneamente capturando particulates e emperramento um componente soluble aos meios do filtro do silicone. O componente soluble encadernado do interesse eluted subseqüentemente dos meios do filtro do silicone. Em uma incorporação da invenção, os materiais soluble não desejados são capturados pelos meios tratados do filtro do silicone e o componente desejado do interesse é recuperado do flu-através de. Em uma outra incorporação da invenção, os componentes diferentes do interesse são recuperados do eluate e flu-através de. Os meios tratados preferidos do filtro do silicone são cinza do hull do arroz ou terra diatomaceous silane-tratada com grupo quarternary funcional do ammonium ou grupo funcional do sulphonate. Os particulates apropriados para a invenção atual, para o exemplo, são microorganisms.

 
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