Nucleic acids encoding phosphorylated fusion proteins

   
   

Modified proteins, modified interferons .alpha.'s and .beta.'s, phosphorylated modified proteins and DNA sequences encoding the above, applications and uses thereof. Modified phosphorylated Hu-IFN-.alpha.-like proteins are provided which carry an identifiable label such as a radio-label. Corresponding phosphorylatable, Hu-IFN-.alpha.-like proteins which contain a putative phosphorylation site. DNA sequences which encode a Hu-IFN-.alpha.-like protein and contain a sequence encoding a putative phosphorylatable site. Appropriate expression vectors are used to transform compatible host cells of various microorganisms, such as E. coli. Numerous uses for the phosphorylated proteins are disclosed.

As proteínas modificadas, os alpha.'s e os beta.'s modificados dos interferons, as proteínas modificadas phosphorylated e o DNA arranjam em seqüência codificar o acima, as aplicações e os usos disso. As proteínas phosphorylated modificadas de Hu-IFN-.alpha.-like são fornecidas que carregam uma etiqueta identifiable tal como rádio-etiquetam. Corresponder phosphorylatable, proteínas de Hu-IFN-.alpha.-like que contêm um local putative do phosphorylation. Seqüências do DNA que codificam uma proteína de Hu-IFN-.alpha.-like e contêm uma seqüência que codifica um local phosphorylatable putative. Os vetores apropriados da expressão são usados transformar pilhas de anfitrião compatíveis de vários microorganisms, tais como E. coli. Os usos numerosos para as proteínas phosphorylated são divulgados.

 
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