Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction

   
   

A purified DNA polymerase exhibiting reverse transcriptase activity in the presence of magnesium ions and/or manganese ions having reduced or no 5'-3'-exonuclease activity and substantially no RNaseH activity and obtainable from Carboxydothermus hydrogenoformans.

Очищенная полимераза ДНАА exhibiting обратная деятельность при transcriptase in the presence of уменьшая ионы магния and/or ионы марганца или никакая работа 5'-3'-exonuclease и substantially no деятельность при RNaseH и достижимо от hydrogenoformans Carboxydothermus.

 
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