Protein purification

   
   

A method for purifying proteins by Protein A chromatography is described which comprises the steps of: (a) adsorbing the protein to Protein A immobilized on a solid phase comprising silica or glass; (b) removing contaminants bound to the solid phase by washing the solid phase with a hydrophobic electrolyte solvent; and (c) recovering the protein from the solid phase.

Um método para purifying proteínas pelo chromatography de Proteína Um é descrito de que compreende as etapas: (a) adsorbing a proteína à proteína A immobilized em uma fase contínua que compreende o silicone ou o vidro; (b) removendo os contaminadores limita à fase contínua lavando a fase contínua com um solvente hydrophobic do eletrólito; e (c) recuperando a proteína da fase contínua.

 
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