Two-chain high molecular weight kininogen, and peptide analogs thereof
having homology to sites within kininogen domain 5, are potent inhibitors
of angiogenesis. The peptides have the formula X.sub.1
-His-Lys-X-Lys-X.sub.2 wherein
X is any amino acid,
X.sub.1 is from zero to twelve amino acids, more preferably from zero to
six amino acids, most preferably from zero to three amino acids, and
X.sub.2 is from zero to twelve amino acids, more preferably from zero to
six amino acids, most preferably from zero to three amino acids.
X is preferably an amino acid having a nonpolar side chain, or a polar side
chain which is uncharged at pH 6.0 to 7.0. X is most preferably Asn, Phe
or His. Methods of inhibiting endothelial cell proliferation and
angiogenesis are provided.
el kininogen alto del peso molecular de la Dos-cadena, y los análogos del peptide de eso que tienen homología a los sitios dentro del dominio 5 del kininogen, son inhibidores potentes del angiogenesis. Los peptides tienen el fórmula X.sub.1 - His-Lys-X-Lys-X.sub.2 en donde X es cualquier aminoácido, X.sub.1 es a partir cero a doce aminoácidos, preferiblemente a partir cero a seis aminoácidos, preferiblemente a partir cero a tres aminoácidos, y X.sub.2 es a partir cero a doce aminoácidos, preferiblemente a partir cero a seis aminoácidos, preferiblemente a partir cero a tres aminoácidos. X es preferiblemente un aminoácido que tiene una cadena lateral no polar, o una cadena lateral polar que es uncharged en pH 6.0 a 7.0. X es preferiblemente Asn, Phe o sus métodos de inhibir la proliferación y el angiogenesis endothelial de la célula se proporcionan.
