Transfer method for specific cellular localization of nucleic acids

   
   

The present invention relates to a novel method of genetic modification, wherein a nucleic acid of interest is transferred across a biological membrane, and/or directed to a specific location within or on a cell, by use of a synthetic transport entity. The transport entity according to the invention is new as such and produced by coupling a functional element (FE), such as a nuclear localization signal (NLS), an antennapedia peptide of a protein comprising both membrane translocation and nuclear transport properties, to a binding element (BE), such as a peptide nucleic acid (PNA), preferably separated by a linker molecule, which combination is then hybridized to a BE target sequence present on a carrier, which also includes the nucleic acid of interest. The present nucleic acid of interest may for example be a gene encoding a peptide, a protein or an RNA, or any other nucleic acid useful in genetic recombination events.

 
Web www.patentalert.com

< Adenovirus vectors for the transfer of foreign genes into cells of the central nervous system particularly in brain

< Newcastle disease virus infectious clones, vaccines and diagnostic assays

> MDA-7 nucleic acid molecules and pharmaceutical compositions thereof

> Maize metallothionein gene and promoter

~ 00108