A device for utilizing a non-gel self-assembled nano-feature array molecular sieve for analyzing molecules is provided. The molecular sieve device comprises an ordered array of self-assembled nano-features which function as a molecular sieve to separate molecules based on a suitable characteristic. A system for integrating the non-gel ordered self-assembled nano-feature array molecular sieve of this invention into a device for separating molecules based on a characteristic and a method for separating a wide range of molecules using the non-gel ordered self-assembled nano-feature array molecular sieve of the invention are also provided.