This invention provides methods of generating cells that stably replicate sub-genomic virus replicons. This invention also provides methods of generating cells that have disabled PKR activity and that stably replicate HCV sub-genomic replicons. The invention also provides methods of using the cells of the invention to screen for compounds that modulate viral RNA replication, including HCV RNA replication.

Esta invenção fornece métodos de gerar as pilhas que replicate estàvel replicons secundários-genomic do vírus. Esta invenção fornece também métodos de gerar as pilhas que incapacitaram a atividade de PKR e que replicate estàvel replicons secundários-genomic de HCV. A invenção fornece também métodos de usar as pilhas da invenção selecionar para os compostos que modulam o replication viral do RNA, including o replication do RNA de HCV.