The invention is directed to the culture of cells, and particularly chondrocytes for purpose of tissue replacement. The cells are cultured on polymer constructs. Integren expression is used as a measure of chondrocyte viability. Chondrocytes are obtained from the knee, nose and ankle cartilage. Mechanical strain is used to propagate chondrocytes, chitosan and arabinogalactanchitosan are used as scaffolds. Progenitor, pluripotential, stem and mesenchymal cells are operative in this invention.

La invención se dirige a la cultura de células, y particularmente a los chondrocytes para el propósito del reemplazo del tejido fino. Las células se cultivan en construcciones del polímero. La expresión de Integren se utiliza como medida de viabilidad del chondrocyte. Chondrocytes se obtiene del cartílago de la rodilla, de la nariz y del tobillo. La tensión mecánica se utiliza para propagar chondrocytes, chitosan y arabinogalactanchitosan se utilizan como andamios. El progenitor, pluripotential, el vástago y las células mesenchymal son operativos en esta invención.