The present invention relates to a novel antibiotic-producing and
metabolite-producing Bacillus subtilis strain that exhibits insecticidal,
antifungal and antibacterial activity. The supernatant of this novel
strain contains effective insecticidal, antifungal and antibacterial
agents. Also included in the invention is a solvent extractable, small
molecular weight (<10,000 daltons) corn rootworm-active metabolite
produced in the supernatant. Also included in the invention are methods of
protecting or treating plants from fungal and bacterial infections and
corn rootworm infestations comprising the step of applying to the plant an
effective amount of the antibiotic/metabolite-producing novel Bacillus
subtilis strain, the antibiotic/metabolite produced by the novel Bacillus
subtilis strain or a combination thereof, optionally further comprising
another antibiotic-producing bacterial strain and/or a chemical pesticide.
The invention also includes methods of preventing or treating fungal and
bacterial infections using whole broth cultures or supernatants obtained
from cultures of the novel Bacillus subtilis stain alone or in combination
with chemical pesticides and/or other biocontrol agents. The invention
also includes novel antifungal and antibacterial compounds designated
agrastatins and a novel combination comprising an A-type iturin, a
plipastatin, a surfactin and an agrastatin. Methods of treating or
protecting plants from fungal and bacterial infections and corn rootworm
infestations comprising administering the novel agrastatins and the novel
combination comprising an A-type iturin, a plipastatin, a surfactin and an
agrastatin are provided. Further provided is a lipopeptide extract
isolated from strain AQ713 with insecticidal activity and a surfactin
lipopeptide isolated from strain AQ713 with insecticidal activity.
La actual invención se relaciona con una novela la tensión del bacilo subtilis antibio'tico-que produce y metabolite-que produce que exhibe actividad insecticida, antihongos y anti-bacteriana. El supernatant de esta tensión de la novela contiene agentes insecticidas, antihongos y anti-bacterianos eficaces. También se incluye en la invención un peso molecular extractable, pequeño solvente (
