Methods for preparing colloidal metal nanoparticles, in which seed colloids are added to a solution of reductant mixed with a solution containing a source of metal ions, include a method in which the seeds are colloidal gold nanoparticles, the source of gold ions is HAuCl.sub.4, and the reductant is NH.sub.2 OH. SERS substrates are prepared by combining a colloidal gold monolayer with a solution containing a source of metal ions and a reductant such as NH.sub.2 OH.

 
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