A physiologically active extract comprising an ethyl acetate-soluble ingredient of an indigo plant, which is obtainable by soaking a raw indigo plant in an organic solvent used for extraction. The ethyl acetate-soluble ingredient includes tryptanthrin, 3,5,4'-trihydroxy-6,7-methylenedioxy-flavone, kaempferol, 3,5,7,4'-tetrahydroxy-6-methoxy-flavone, gallic acid, caffeic acid, indirubin, pheophorbide a, and methylpheophorbide a.