This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an .alpha.2,3- and an .alpha.2,8-activity. A .beta.1,4-GalNAc transferase and a .beta.1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

Этот вымысел обеспечивает prokaryotic glycosyltransferases, включая sialyltransferase bifunctional которое имеет и alpha.2,8-de4tel6nost6 alpha.2,3- и. Трансфераза beta.1,4-GalNAc и beta.1,3-$$ET-beta.1,3-galactosyltransferase также обеспечены вымыслом, как другие glycosyltransferases и энзимы, котор включили в синтез lipooligosaccharide (LOS). Glycosyltransferases можно получить от, например, вид campylobacter, включая jejuni C. В дополнительных воплощениях, вымысел обеспечивает нуклеиновые кислоты которые шифруют glycosyltransferases, также,как векторы выражения и ведущие ячейки для выражать glycosyltransferases.

 
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