Antisense compounds, compositions and methods are provided for modulating the expression of Casein kinase 2-alpha prime. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding Casein kinase 2-alpha prime. Methods of using these compounds for modulation of Casein kinase 2-alpha prime expression and for treatment of diseases associated with expression of Casein kinase 2-alpha prime are provided.