The invention relates to the purification, crystallization of and structure of hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase. Also, crystallization conditions for NS5B are provided. Further, the atomic coordinates for the NS5B protein are disclosed. Examples of its use for the determination of the three-dimensional atomic structures of HCV NS5B or HCV NS5B in complex with substrates or substrate analogs or inhibitors are also provided.

A invenção relaciona-se ao purification, à cristalização de e à estrutura do polymerase RNA-dependente do RNA do vírus do hepatitis C (HCV) NS5B. Também, as condições da cristalização para NS5B são fornecidas. Mais mais, as coordenadas atômicas para a proteína de NS5B são divulgadas. Os exemplos de seu uso para a determinação das estruturas atômicas tridimensionais de HCV NS5B ou de HCV NS5B no complexo com carcaças ou analogs da carcaça ou inibidores são fornecidos também.

 
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