The invention relates to oligonucleotides labeled with an energy transfer acceptor useful in conjunction with fluorescent nucleic acid stains. The resulting oligonucleotides are useful for decreasing background fluorescence during amplification assays and in ligation assays, and for detecting hybridization.

A invenção relaciona-se aos oligonucleotides etiquetados com um aceitador de transferência de energia útil conjuntamente com manchas fluorescentes do ácido nucleic. Os oligonucleotides resultantes são úteis para diminuir o fluorescence do fundo durante assays do amplification e em assays do ligation, e para detectar o hybridization.